Circularized Visualisation of Genetic Interactions
نویسندگان
چکیده
Next Generation Sequencing (NGS) has been a powerful tool to investigate gene networks in biological sciences [1]. Visualisation of data produced by NGS is essential for the interpretation of the findings by biological scientists. Here we describe a workflow to image findings from a NGS sequencing methodology to investigate gene expression that can be visualised with Circus software [2]. Visualisation of these processes has provided biological scientists with valuable interpretation of high throughput data and identification of new transcripts.
منابع مشابه
Heterochromatin position effects on circularized sex chromosomes cause filicidal embryonic lethality in Drosophila melanogaster.
Some circularized X-Y chromosomes in Drosophila melanogaster are mitotically unstable and induce early embryonic lethality, but the genetic basis is unknown. Our experiments suggest that a large region of X-linked satellite DNA causes anaphase bridges and lethality when placed into a new heterochromatic environment within certain circularized X-Y chromosomes. These results reveal that repetitiv...
متن کاملIdentification of Genetic Polymorphism Interactions in Sporadic Alzheimer’s Disease Using Logic Regression
Objectives: Genetic polymorphism interactions are among the important factors in affliction with complex diseases like Alzheimer’s disease. The important goal of genetic association studies is to identify a combination of polymorphisms and measure their importance in increasing the risk of occurrence of such diseases. In this study, feature selection approach of logic regression was used to ide...
متن کاملComplete Circularized Genome Sequences of Four Strains of Elizabethkingia anophelis, Including Two Novel Strains Isolated from Wild-Caught Anopheles sinensis
We provide complete circularized genome sequences of two mosquito-derived Elizabethkingia anophelis strains with draft sequences currently in the public domain (R26 and Ag1), and two novel E. anophelis strains derived from a different mosquito species, Anopheles sinensis (AR4-6 and AR6-8). The genetic similarity of all four mosquito-derived strains is remarkable.
متن کاملThe Infection Efficiency and Replication Ability of Circularized HBV DNA Optimized the Linear HBV DNA in Vitro and in Vivo
Studies on molecular mechanisms of the persist infection of hepatitis B virus have been hampered by a lack of a robust animal model. We successfully established a simple, versatile, and reproducible HBV persist infection model in vitro and in vivo with the circularized HBV DNA. The cells and mice were transfected or injected with circularized HBV DNA and pAAV/HBV1.2, respectively. At the indica...
متن کاملA recombination function essential to the growth of bacteriophage P22.
Mutants of the Sahrwneliu phage P22 have been isolated which cannot grow after infection of recombination-deficient (ret-) hosts. These mutants (designated erffor essential recombination function) are defective in a phage-specified recombination system, as shown by twoand three-factor crosses carried out in WC + and rethosts. Four erf mutants examined in detail fall into one complementation gro...
متن کامل